Nitric Oxide Research Papers

論文連接


Byung Mun Park a,1, Seung Ah Cha a,1, Hye Yoom Kim b, Dae Kil Kang b,
Kuichang Yuan c, Hyunsoo Chun d, SooWan Chae e, Suhn Hee Kim a,*
a Department of Physiology, Chonbuk National University Medical School, Jeonju, Republic of Korea
b Department of Physiology,Wonkwang University Oriental Medicine, Iksan, Republic of Korea
c Department of Internal Medicine, Yanbian University, China
d HtO Life Co., LTD,Wanju-gun, Republic of Korea
e Clinical Trial Center for Functional Foods, Chonbuk National University Hospital, Jeonju, Republic of Korea

A B S T R A C T
Garlic (Allium sativum L.) has long been used as an anti-hypertensive. The study investigated the effect of fermented garlic extract (Fgarlic) on systolic blood pressure (SBP) and its molecular basis using spontaneously hypertensive rats (SHRs). Fgarlic contained high content of stable nitrite (0.975 mg nitrite/ml). Acute feeding of different amounts (0.3, 0.6, and 0.9 ml) of concentrated Fgarlic (9.75 mg nitrite/ml) reduced SBP dose-dependently. Chronic feeding of Fgarlic (containing 27 mg nitrite/day) for 12 days reduced SBP with increased expressions of eNOS and PKG proteins in aortic tissues, which were attenuated by a soluble guanylyl cyclase (sGC) inhibitor. The relaxation responses of thoracic aorta to acetylcholine and sodium nitroprussside were improved in SHRs fed Fgarlic. These results suggest that nitrite in Fgarlic, which converts to NO in the body, functions as an anti-hypertensive molecule and its effect is mediated through sGC-cGMP-PKG pathway.
© 2016 Elsevier Ltd. All rights reserved.


Byung Mun Park a, Hyunsoo Chun d, Soo Wan Chae b,c, Suhn Hee Kim a,⇑
a Department of Physiology, Research Institute for Endocrine Sciences, Chonbuk National University Medical School, Jeonju 54907, Republic of Korea
b Department of Pharmacology, Research Institute for Endocrine Sciences, Chonbuk National University Medical School, Jeonju 54907, Republic of Korea
c Clinical Trial Center for Functional Foods, Chonbuk National University Hospital, Jeonju 54907, Republic of Korea
d HtO Life Co., LTD, Wanju-gun 565-864, Republic of Korea


A B S T R A C T
This study investigated the effect of fermented garlic extract (FGE) on pulmonary hypertension in monocrotaline (MCT)-treated rats. Sprague-Dawley rats were subcutaneously injected with either 50 mg/kg MCT or a vehicle 3 days after the FGE feeding (0.97 mg nitrite/ml/day) was initiated. MCT treatment increased the weight, systolic pressure, and atrial natriuretic peptide concentration in the right ventricle but not in the left ventricle. FGE feeding attenuated these effects as well as the endothelial damage and medial hypertrophy of the pulmonary arterioles and the pulmonary fibrosis induced by MCT. These FGE effects were blocked by a soluble guanylyl cyclase (sGC) inhibitor. Increases in VCAM-1 and MMP-9 protein expressions, and decreases in PKG and eNOS protein expressions in the lung of MCT rats were attenuated by FGE feeding. These findings suggest that FGE ameliorates MCT-induced pulmonary hypertension by decreasing the inflammatory reaction via the NO–sGC–PKG pathway.
© 2017 Elsevier Ltd. All rights reserved.


Young Ju Lee a,1, Doyeon Lee b,1, So Min Shin c, Jin Sun Lee c, Hyun Soo Chun d, Fu-Shi Quan e, Jae Ho Shin c, Gi-Ja Lee a,b,⇑
a Department of Biomedical Engineering, College of Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea
b Department of Medical Engineering, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea
c Department of Chemistry, College of Natural Science, Kwangwoon University, 20 Kwangwoon-ro, Nowon-gu, Seoul 01897, Republic of Korea
d Department of National Cosmetics Science, Sunchon National University, 255 Jungang-ro, Suncheon-si, Jeollanam-do 57922, Republic of Korea
e Department of Medical Zoology, College of Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea

A B S T R A C T
In this study, we investigated the cardioprotective effects of fermented garlic extract (FGE) against ischemia-reperfusion (IR) injury in H9c2 cells and myocardium. FGE used in this study is rich in nitrite (1.42 mg/ml of FGE) and reduced glutathione (GSH, 3.23 mg/ml of FGE). We compared the effectiveness of FGE against HR–induced oxidative stress with that of nitrite, GSH, and a mixture of nitrite and GSH (nitrite + GSH). In addition, we utilized fluorinated xerogel-derived oxygen microsensors to measure the changes in oxygen level (pO2) of the myocardium during myocardial IR by FGE. As a result, the pO2 in the FGE group restored to pre-ischemic level (75.1 ± 36.9%) at reperfusion period; however, that of the control group did not increase throughout reperfusion period (2.0 ± 1.7%, p < 0.05). Therefore, pretreatment with FGE might be helpful as a therapeutic strategy for preventing H9c2 cells and myocardium from IR injury.
©  2017 Elsevier Ltd. All rights reserved.



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